Luminol has an emission half-life of less than a minute. Western blotting protein standards can be used for both fluorescent visualization and colormetric or chemiluminescent immunodetection on western blots. Use these standards for both fluorescent visualization and colorimetric or chemiluminescent immunodetection on western blots. The type of enhancer used can also increase the signal duration. Unstained protein ladders are optimized for Coomassie and zinc staining. For example, the enhancers used in Bio-Rad Clarity Max ECL substrate maximizes detection sensitivity to enable detection to the low femtogram amounts of target.

There are a number of different enhancers available, some of which can increase the signal by as much as a 1,000-fold, making ECL more sensitive than other common detection systems such as conversion of substrates to colored precipitates. This enhancement of a luminol-based signal is commonly referred to as enhanced chemiluminescence (ECL). Increased brightness makes it easier to: Identify target proteins on the gel or blot. Improved Dual Color Standards are brighter than the competition on both gels and blots. However, luminol emits light only weakly, so enhancers are added to the reaction to increase the signal. Increased overall brightness provides visual robustness throughout the entire protein electrophoresis and western blotting workflow. This reaction emits light at 428 nm and this light signal can be captured on X-ray film or by a digital imager. The secondary antibody used in western blotting is conjugated to the horseradish peroxidase (HRP) enzyme which reacts with the HRP substrate luminol. Improve efficiency with Bio-Rads 1-D and 2-D electrophoresis systems, gels, cells, standards, buffers, and reagents for protein electrophoresis and blotting. View ECL Blotting Substrates ECL Substrates for High-Sensitivity Western Blot DetectionĬhemiluminescent assays depend on the emission of light as a product of a chemical reaction and are commonly used for the detection of proteins on western blots. Find information on protein visualization and quantitation methods, gel and blot imaging instrumentation, and image analysis software. Clarity and Clarity Max are compatible with any horseradish peroxidase (HRP) conjugate and ideal for all western blots with both digital and film-based imaging systems.

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